1)Alcohol swab was used to clean the third finger. Never use thumb for adult and children. Firm strokes was used to remove dirt grease from the ball of the finger.
2)A white tile was wiped with 70 % ethanol using dry cotton wool to avoid contamination.
3)The finger was being dried with a clean cotton wool then firm strokes was used to stimulate blood circulation.
4)The ball of the finger was punctured with a sterile lancet. Then, a gentle pressure was applied on the finger. First drop of blood was expressed and being wiped away with dry cotton wool. Make sure the finger had no strands of cotton remain on it.
5)A single drop of blood was collected using disposible plastic Pasteur pipette and was put into respective square of the tile.
6)Next, a drop of Anti-Sera (A


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